Biology Biotechnology PMDC Conceptual Practice
PMDC Verified Question 1 of 65
Synthesis of cDNA from mRNA is catalyzed by:
A
DNA polymerase III
B
Restriction endonuclease
C
Reverse transcriptase
D
DNA ligase
Tap any option to test your recall and reveal the step-by-step Propolis autopsy.

Propolis Cognitive Error Autopsy

Official Correct Choice:
Option C: Reverse transcriptase
Concept:

Reverse transcription is the process of synthesizing complementary DNA (cDNA) from a single-stranded messenger RNA (mRNA) template.

Solution:

Reverse transcriptase is an RNA-dependent DNA polymerase enzyme originally isolated from retroviruses. It synthesizes a single-stranded cDNA copy from a mature eukaryotic mRNA molecule.

Why other options are incorrect:

  • DNA polymerase III: The primary enzyme responsible for prokaryotic genomic DNA replication, using DNA as a template.


  • Restriction endonuclease: Bacterial enzymes that cleave double-stranded DNA at specific palindromic recognition sites.


  • DNA ligase: Catalyzes the formation of phosphodiester bonds to seal nicks between adjacent DNA fragments.

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