Biology Biotechnology PMDC Conceptual Practice
PMDC Verified Question 7 of 65
The molecular glue used to join foreign DNA into a vector is:
A
Restriction enzyme
B
DNA polymerase
C
RNA primase
D
DNA ligase
Tap any option to test your recall and reveal the step-by-step Propolis autopsy.

Propolis Cognitive Error Autopsy

Official Correct Choice:
Option D: DNA ligase
Concept:

Enzymatic ligation of sugar-phosphate backbones in recombinant DNA technology.

Solution:

DNA ligase acts as a molecular glue by catalyzing the formation of covalent phosphodiester bonds between adjacent \( 3' \text{-OH} \) and \( 5' \text{-PO}_4 \) termini of DNA fragments.

Why other options are incorrect:

  • Restriction enzyme: Acts as molecular scissors to cut DNA molecules at specific target sites.


  • DNA polymerase: Synthesizes new complementary DNA strands by adding deoxynucleotides to a pre-existing primer.


  • RNA primase: Synthesizes short RNA primers required to initiate DNA replication.

Quality & Fidelity Assurance: Every question on BeambePrep is rigorously curated against the official PMDC syllabus with zero filler, zero out-of-syllabus content, and zero typos. When an authentic past paper originally contained a historical mistake or ambiguity from the examining board (such as UHS or NUMS), BeambePrep faithfully reflects the original paper while detailing the nuance and scientific consensus in the autopsy above.

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