Biology Biotechnology PMDC Conceptual Practice
PMDC Verified Question 10 of 65
In a PCR cycle, DNA denaturation is carried out at:
A
\( 37^\circ \text{C} \)
B
\( 54^\circ \text{C} \)
C
\( 94^\circ \text{C} \)
D
\( 72^\circ \text{C} \)
Tap any option to test your recall and reveal the step-by-step Propolis autopsy.

Propolis Cognitive Error Autopsy

Official Correct Choice:
Option C: \( 94^\circ \text{C} \)
Concept:

Thermal steps of the Polymerase Chain Reaction (PCR).

Solution:

Denaturation occurs at high temperatures (typically \( 94^\circ \text{C} - 96^\circ \text{C} \)), breaking the hydrogen bonds between complementary base pairs to yield single-stranded DNA templates.

Why other options are incorrect:

  • \( 37^\circ \text{C} \): Standard physiological incubation temperature for human enzymes, far too low to denature DNA.


  • \( 54^\circ \text{C} \): Represents the typical annealing temperature range (\( 50^\circ \text{C} - 65^\circ \text{C} \)) for primer binding.


  • \( 72^\circ \text{C} \): The optimal extension temperature for Taq DNA polymerase synthesis.

Quality & Fidelity Assurance: Every question on BeambePrep is rigorously curated against the official PMDC syllabus with zero filler, zero out-of-syllabus content, and zero typos. When an authentic past paper originally contained a historical mistake or ambiguity from the examining board (such as UHS or NUMS), BeambePrep faithfully reflects the original paper while detailing the nuance and scientific consensus in the autopsy above.

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