Biology Biotechnology PMDC Conceptual Practice
PMDC Verified Question 19 of 65
To obtain virus-free plants, the tissue culture method used is:
A
Protoplast fusion
B
Anther culture
C
Callus culture
D
Meristem culture
Tap any option to test your recall and reveal the step-by-step Propolis autopsy.

Propolis Cognitive Error Autopsy

Official Correct Choice:
Option D: Meristem culture
Concept:

Eradication of plant systemic viruses via shoot tip explant culture.

Solution:

The shoot apical meristem is continuously dividing and lacks functional vascular connections, making it free of systemic viruses. Culturing apical meristems yields virus-free plant lines.

Why other options are incorrect:

  • Protoplast fusion: Used to produce somatic hybrids between different plant species by fusing naked plant cells.


  • Anther culture: Used to generate haploid plants from microspores for plant breeding.


  • Callus culture: Cultures unorganized mass of parenchymatous cells; infected mother tissue transfers viral particles to the callus.

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