Biology Biotechnology PMDC Conceptual Practice
PMDC Verified Question 4 of 65
Which restriction enzyme produces blunt ends upon cutting DNA?
A
EcoRI
B
HindIII
C
EcoRV
D
BamHI
Tap any option to test your recall and reveal the step-by-step Propolis autopsy.

Propolis Cognitive Error Autopsy

Official Correct Choice:
Option C: EcoRV
Concept:

Restriction enzymes cut DNA to generate either staggered (sticky/cohesive) ends or straight (blunt) flush ends.

Solution:

EcoRV cleaves double-stranded DNA at the center of its recognition sequence \( 5' \text{-GAT|ATC-} 3' \), generating non-overlapping, flush-ended (blunt) double-stranded DNA fragments.

Why other options are incorrect:

  • EcoRI: Cleaves asymmetrically between G and A in \( 5' \text{-G|AATTC-} 3' \) to produce \( 5' \) sticky overhangs.


  • HindIII: Cleaves asymmetrically at \( 5' \text{-A|AGCTT-} 3' \) producing cohesive sticky ends.


  • BamHI: Cleaves asymmetrically at \( 5' \text{-G|GATCC-} 3' \) generating sticky overhangs.

Quality & Fidelity Assurance: Every question on BeambePrep is rigorously curated against the official PMDC syllabus with zero filler, zero out-of-syllabus content, and zero typos. When an authentic past paper originally contained a historical mistake or ambiguity from the examining board (such as UHS or NUMS), BeambePrep faithfully reflects the original paper while detailing the nuance and scientific consensus in the autopsy above.

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