Biology Biotechnology PMDC Conceptual Practice
PMDC Verified Question 5 of 65
The plasmid pBR322 contains selectable marker genes for resistance against:
A
Ampicillin and Tetracycline
B
Streptomycin and Penicillin
C
Kanamycin and Chloramphenicol
D
Erythromycin and Neomycin
Tap any option to test your recall and reveal the step-by-step Propolis autopsy.

Propolis Cognitive Error Autopsy

Official Correct Choice:
Option A: Ampicillin and Tetracycline
Concept:

Selectable marker genes in cloning vectors allow the screening and selection of transformed host cells.

Solution:

The engineered plasmid pBR322 carries two distinct antibiotic resistance marker genes: ampR (conferring resistance to ampicillin) and tetR (conferring resistance to tetracycline).

Why other options are incorrect:

  • Streptomycin and Penicillin: These antibiotic markers are not present on the standard pBR322 vector map.


  • Kanamycin and Chloramphenicol: Found in other specialized plasmids (such as pUC or pACYC vectors), not pBR322.


  • Erythromycin and Neomycin: Used in eukaryotic selection systems or other specific bacterial vectors, not pBR322.

Quality & Fidelity Assurance: Every question on BeambePrep is rigorously curated against the official PMDC syllabus with zero filler, zero out-of-syllabus content, and zero typos. When an authentic past paper originally contained a historical mistake or ambiguity from the examining board (such as UHS or NUMS), BeambePrep faithfully reflects the original paper while detailing the nuance and scientific consensus in the autopsy above.

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